China Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit baetsi le barekisi |Liming Bio

Novel Coronavirus (SARS-CoV-2) Multiplex Real-Nako PCR Kit

Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit Featured Image

Tlhaloso e Khutšoanyane:

REF	500190	Tlhaloso	96 Liteko / Lebokose
Molao-motheo oa ho lemoha	PCR	Mehlala	Nasal / Nasopharyngeal swab
Tšebeliso e reriloeng	Sena se reretsoe ho sebelisoa ho fihlela tlhahlobo ea boleng ba RNA ea SARS-CoV-2 e ntšitsoeng ho nasopharyngeal swabs, oropharyngeal swabs, sputum le BALF ho tsoa ho bakuli ka kopanelo le FDA/CE IVD system ea ho ntšoa le sethala se khethiloeng sa PCR se thathamisitsoeng ka holimo.

Setsi sena se reretsoe ho sebelisoa ke basebetsi ba koetlisitsoeng laboratoring

Re romelle lengolo-tsoibila Khoasolla joalo ka PDF

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

PCR kit ena e hlokolosi haholo, e seng e loketse ho sebelisoa e fumaneha ka mokhoa oa lyophilized (mokhoa oa ho omisa ka leqhoa) bakeng sa polokelo ea nako e telele.Thepa e ka tsamaisoa le ho bolokoa mocheso oa kamore 'me e tsitsitse selemo se le seng.Tube e 'ngoe le e' ngoe ea premix e na le li-reagents tsohle tse hlokahalang bakeng sa ho holisa PCR, ho kenyeletsoa Reverse-transcriptase, Taq polymerase, primers, probes, le dNTPs substrates.E hloka feela ho eketsa metsi a hloekisitsoeng a 13ul le template ea 5ul e nkiloeng ea RNA, ebe e ka tsamaisoa le ho holisoa lisebelisoa tsa PCR.

Mochini oa qPCR o lokela ho fihlela litlhoko tse latelang:
1. Fit 8 strip PCR tube volume 0,2 ml
2. Ho ba le mecha e fetang 4 ea ho lemoha:

Channel	Ho hlasimolla (nm)	Phallo (nm)	Li-dyes tse lekantsoeng pele
1.	470	525	FAM, SYBR Green I
2	523	564	VIC, HEX, TET, JOE
3.	571	621	ROX, TEXAS-RED
4	630	670	CY5

Li-platform tsa PCR:
7500Real-Time PCR System, Biorad CF96, iCycler iQ™ Real-Time PCR Detection System, Stratagene Mx3000P, Mx3005P

Bothata ba lipalangoang tse batang tsa Novel Coronavirus nucleic acid discovery reagent
Ha li-reagents tse tloaelehileng tsa ho lemoha ha li-nucleic acid li tsamaisoa sebakeng se selelele, (-20±5) ℃ polokelo le lipalangoang tse batang lia hlokahala ho netefatsa hore bioactive ea enzyme ho li-reagents e lula e sebetsa.Ho etsa bonnete ba hore mocheso o fihla boemong bo tloaelehileng, ho hlokahala lik'hilograma tse 'maloa tsa leqhoa le omileng bakeng sa lebokose le leng le le leng la nucleic acid test reagent esita le ka tlase ho 50g, empa e ka nka matsatsi a mabeli kapa a mararo feela.Ka pono ea ts'ebetso ea indasteri, boima ba 'nete ba li-reagents tse fanoeng ke bahlahisi bo ka tlase ho 10% (kapa ka tlase ho boleng bona) ba setshelo.Boholo ba boima bo tsoa ho leqhoa le omileng, lipakete tsa leqhoa le mabokose a foam, kahoo litšenyehelo tsa lipalangoang li phahame haholo.

Ka Hlakubele 2020, COVID-19 e ile ea qala ho ata ka bongata kantle ho naha, mme tlhoko ea Novel Coronavirus nucleic sensor reagent e ile ea eketseha haholo.Ho sa tsotellehe litšenyehelo tse phahameng tsa ho romela li-reagents ka har'a ketane e batang, bahlahisi ba bangata ba ntse ba ka e amohela ka lebaka la bongata bo bongata le phaello e phahameng.

Leha ho le joalo, ka ntlafatso ea maano a naha a thomello ea thepa bakeng sa lihlahisoa tse khahlanong le seoa, hammoho le ntlafatso ea taolo ea naha holim'a phallo ea batho le thepa, ho na le katoloso le ho hloka botsitso nakong ea lipalangoang tsa li-reagents, e leng se bakileng mathata a hlahelletseng a lihlahisoa. ka lipalangoang.Nako e atolositsoeng ea lipalangoang (nako ea lipalangoang e ka bang halofo ea khoeli e tloaelehile haholo) e lebisa ho hloleheng ha sehlahisoa khafetsa ha sehlahisoa se fihla ho moreki.Sena se tšoentse likhoebo tse ngata tse romellang li-nucleic acid.

Theknoloji ea Lyophilized bakeng sa reagent ea PCR e thusitse ho tsamaisa thepa ea Novel Coronavirus nucleic acid e lemohang lefats'e ka bophara.

Li-reagents tsa PCR tse nang le lyophilized li ka tsamaisoa le ho bolokoa mocheso oa kamore, o ke keng oa fokotsa litšenyehelo tsa lipalangoang feela, empa hape o qoba mathata a boleng a bakoang ke mokhoa oa lipalangoang.Ka hona, lyophilizing reagent ke eona tsela e molemohali ea ho rarolla bothata ba lipalangoang tsa kantle ho naha.

Lyophilization e kenyelletsa ho hatsetsa tharollo hore e be boemong bo tiileng, ebe e fokotsa le ho arola mouoane oa metsi tlas'a boemo ba vacuum.Solute e omisitsoeng e sala ka har'a setshelo se nang le sebopeho le mosebetsi o tšoanang.Ha ho bapisoa le li-reagents tse tloaelehileng tsa mokelikeli, karolo e felletseng ea lyophilized Novel Coronavirus nucleic acid e lemohang reagent e hlahisoang ke Liming Bio e na le litšobotsi tse latelang:

Ho tsitsisa mocheso o matla haholo:e ka khona ka kalafo ea ho ema ho 56 ℃ bakeng sa matsatsi a 60, 'me morphology le ts'ebetso ea reagent li lula li sa fetohe.
Ho boloka mocheso le lipalangoang tse tloaelehileng:ha ho na tlhoko ea ketane e batang, ha ho na tlhoko ea ho boloka mocheso o tlase pele o koala, lokolla ka botlalo sebaka sa polokelo se batang.
E se e loketse ho sebelisoa:lyophilizing ea likarolo tsohle, ha ho hlokahale ho hlophisoa ha tsamaiso, ho qoba ho lahleheloa ke likarolo tse nang le viscosity e phahameng joalo ka enzyme.
Multiplex targets ka tube e le 'ngoe:sepheo sa ho sibolla se akaretsa mofuta oa "coronavirus" oa ORF1ab, mofuta oa N, S ho qoba ho fetoha ha vaerase.E le ho fokotsa bobe ba bohata, mofuta oa RNase P oa motho o sebelisoa e le taolo ea ka hare, e le ho finyella tlhokahalo ea kliniki ea ho laola boleng ba sampole.