Mokhoa o motle ke ofe?
- Liteko tsa Tlhahlobo ea Ts'oaetso ea SARS-CoV-2
Bakeng sa linyeoe tse netefalitsoeng tsa COVID-19, ho tlalehiloe matšoao a tloaelehileng a kliniki a kenyelletsa feberu, khohlela, myalgia kapa mokhathala.Leha ho le joalo matšoao ana ha se likarolo tse ikhethang tsa COVID-19 hobane matšoao ana a tšoana le a mafu a mang a tšoaelitsoeng ke vaerase joalo ka ntaramane.Hajoale, virus nucleic acid Real-Time PCR (rt-PCR), CT imaging le likarolo tse ling tsa hematology ke lisebelisoa tsa mantlha tsa tlhahlobo ea kliniki ea tšoaetso.Lisebelisoa tse ngata tsa liteko tsa laboratori li entsoe le ho sebelisoa ho lekola mehlala ea bakuli bakeng sa COVID-19 ke CDC ea China.1, US CDC2le lik'hamphani tse ling tse ikemetseng.Teko ea antibody ea IgG/IgM, e leng mokhoa oa tlhahlobo ea serological, le eona e ekelitsoe e le mokhoa oa tlhahlobo ea tlhahlobo ea China ea tlhahlobo le litataiso tsa kalafo bakeng sa lefu la "coronavirus" (COVID-19), le fanoeng ka la 3 Hlakubele.1.Teko ea vaerase ea nucleic acid rt-PCR e ntse e le mokhoa oa hajoale oa tlhahlobo ea tlhahlobo ea COVID-19.
StrongStep®Novel Coronavlrus (SARS-COV-2)Multiplex Real-Time PCR Kit(ho lemoha mefuta e meraro ea lefutso)
Leha ho le joalo lisebelisoa tsena tsa tlhahlobo tsa PCR tsa nako ea 'nete, tse batlang liphatsa tsa lefutso tsa vaerase, mohlala ka linkong, molomong kapa lithakong, li na le mefokolo e mengata:
1) Liteko tsena li na le nako e telele ea ho fetola 'me li rarahane ts'ebetsong;hangata ba nka ka karolelano lihora tse 2 ho isa ho tse 3 ho hlahisa litholoana.
2) Liteko tsa PCR li hloka lilaboratori tse netefalitsoeng, lisebelisoa tse turang le litsebi tse koetlisitsoeng ho sebetsa.
3) Ho na le lipalo tse ling tsa li-negative tsa bohata bakeng sa rt-PCR ea COVID-19.E kanna ea ba ka lebaka la palo e tlase ea kokoana-hloko ea SARS-CoV-2 sepakapakeng se kaholimo sa phefumoloho (Novel coronavirus haholo-holo e tšoaetsa pampitšana e tlase ea phefumoloho, joalo ka pulmonary alveoli) mme tlhahlobo ha e khone ho tseba batho ba fetileng ts'oaetso, ba fola, mme a tlosa kokoana-hloko 'meleng ea bona.
Lipatlisiso ka Lirong Zou et al4e fumane hore bongata ba kokoana-hloko bo ile ba bonoa kapele ka mor'a hore matšoao a qale, 'me bongata ba kokoana-hloko bo fumanoe ka nkong ho feta 'metso le mokhoa oa ho tšolla oa "viral nucleic acid" oa bakuli ba tšoaelitsoeng ke SARS-CoV-2 o tšoana le oa bakuli ba ntaramane.4'me e bonahala e fapane le e bonoang ho bakuli ba tšoaelitsoeng ke SARS-CoV-2.
Yang Pan et al5E ile ea hlahloba lisampole tsa serial (li-swabs tsa 'metso, sekhohlela, moroto le mantle) ho tsoa ho bakuli ba babeli ba Beijing, 'me ba fumana hore bongata ba kokoana-hloko 'metsong le lisampole tsa likhohlela li fihlile matsatsing a 5-6 ka mor'a hore matšoao a qale, lisampole tsa sekhohlela hangata li bonts'a bongata ba kokoana-hloko ho feta. lisampole tsa 'metso.Ha ho RNA ea kokoana-hloko e fumanoeng ka har'a moroto kapa lisampole tsa mantle ho tsoa ho bakuli bana ba babeli.
Tlhahlobo ea PCR e fana ka sephetho se nepahetseng feela ha vaerase e ntse e le teng.Liteko ha li khone ho tseba batho ba kileng ba tšoaetsoa, ba fola, 'me ba tlosa vaerase' meleng ea bona.Ha e le hantle, ke 30% -50% feela e neng e le ntle bakeng sa PCR ho bakuli ba nang le lefu la matšoafo la coronavirus.Bakuli ba bangata ba pale ea "coronavirus" ea "pneumonia" ha ba khone ho fumanoa ka lebaka la tlhahlobo e mpe ea nucleic acid, kahoo ha ba khone ho fumana kalafo e nepahetseng ka nako.Ho tloha khatisong ea pele ho ea ho ea botšelela ea litataiso, ho itšetlehile feela motheong oa ho fumanoa ha liphetho tsa tlhahlobo ea nucleic acid, e leng se ileng sa baka bothata bo boholo ho lingaka. "Mololi oa pele", Dr. Li Wenliang, ngaka ea mahlo Wuhan Central Sepetlele, se shoele.Nakong ea bophelo ba hae, o ile a etsa liteko tse tharo tsa nucleic acid tabeng ea feberu le khohlela, 'me lekhetlo la ho qetela a fumana liphello tse ntle tsa PCR.
Ka mor'a lipuisano tsa litsebi, ho ile ha etsoa qeto ea ho eketsa mekhoa ea tlhahlobo ea serum e le mokhoa o mocha oa ho hlahloba.Leha liteko tsa antibody, tseo hape li bitsoang liteko tsa serological, tse ka netefatsang hore na motho o tšoaelitsoe le kamora hore sesole sa bona sa 'mele se hlakole vaerase e bakang COVID-19.
StrongStep® SARS-COV-2 IgG/IgM Antibody Rapid Test
Teko ea antibody ea IgG/IgM e tla thusa ho fumana ka tsela e ipapisitseng le baahi hore na ke mang ea nang le tšoaetso, hobane linyeoe tse ngata li bonahala li hasana ho tsoa ho bakuli ba asymptomatic ba ke keng ba tsejoa habonolo.Banyalani ba bang Singapore, monna o ile a fumana hore o na le tšoaetso ke PCR, sephetho sa mosali oa hae sa PCR se ne se le sieo, empa liphetho tsa tlhahlobo ea antibody li bonts'a hore o na le li-antibodies, joalo ka monna oa hae.
Litlhahlobo tsa serological li hloka ho netefatsoa ka hloko ho etsa bonnete ba hore li sebetsa ka ts'epo, empa ho li-antibodies tse khahlano le vaerase e ncha.Taba e 'ngoe e ne e le hore ho tšoana lipakeng tsa livaerase tse bakang lefu le matla la ho hema le COVID-19 ho ka lebisa ho ts'ebetsong.IgG-IgM e ntlafalitsoeng ke Xue Feng wang6e ne e nkoa e ka khona ho sebelisoa e le tlhahlobo ea ntlha ea tlhokomelo (POCT), kaha e ka etsoa haufi le bethe ka mali a menoana.Kit e na le kutloisiso ea 88.66% le e ikhethang ea 90.63%.Leha ho le joalo, ho ne ho ntse ho e-na le liphello tse fosahetseng tse fosahetseng le tse fosahetseng.
Phetolelong e ntlafalitsoeng ea China ea tlhahlobo le tataiso ea kalafo bakeng sa lefu le lecha la coronavirus (COVID-19)1, linyeoe tse netefalitsoeng li hlalosoa e le linyeoe tse belaelloang tse fihlelang e 'ngoe ea lintlha tse latelang:
(1) lisampole tsa pampitšana ea ho phefumoloha, mali kapa mantle a fumanoe a le ntle bakeng sa SARS-CoV-2 nucleic acid e sebelisang rt-PCR;
2
(3)Serum novel coronavirus e khethehileng ea IgM antibody le IgG antibody e ne e le ntle;
4
Tlhahlobo le kalafo ea COVID-19
| Tataiso | E phatlalalitsoe | Mekhoa ea tlhahlobo e netefalitsoeng |
| Phetolelo ea 7 | La 3 Hlakubele 2020 | ❶ PCR ❷ NGS ❸ IgM+IgG |
| Phetolelo ea 6 | 18 Hlakola.2020 | ❶ PCR ❷ NGS |
Tshupiso
1. Litaelo tsa tlhahlobo le kalafo ea nova coronavirus pneumonia (teko ea 7, Khomishene ea Naha ea Bophelo bo Botle ea People's Republic of China, e fanoeng ka 3.Mar.2020)
http://www.nhc.gov.cn/yzygj/s7652m/202003/a31191442e29474b98bfed5579d5af95.shtml
2. Lipatlisiso li Sebelisa Feela Protocol ea Nako ea 'Nete ea RT-PCR bakeng sa Boitsebiso ba 2019-nCoV.
https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-detection-instructions.html
3. Singapore e bolela tšebeliso ea pele ea tlhahlobo ea antibody ho latela tšoaetso ea coronavirus
https://www.sciencemag.org/news/2020/02/singapore-claims-first-use-antibody-test-track-coronavirus-infections
4.SARS-CoV-2 Viral Load Lipalong tse ka Holimo tsa Phefumoloho tsa Bakuli ba tšoaelitsoeng la 19 Hlakola, 2020 DOI: 10.1056/NEJMc2001737
5.Viralloads ea SARS-CoV-2 lisampoleng tsa bongaka tsa Lancet Infect Dis 2020 E phatlalalitsoe Marang-rang la 24 Hlakola, 2020 (https://doi.org/10.1016/S1473-3099(20)30113-4)
6. Ts'ebetso ea Ntlafatso le Ts'ebeliso ea Kliniki ea Tlhahlobo e potlakileng ea IgM-IgG e Kopantsoeng ea Antibody bakeng sa SARS-CoV-2.
Tlhahlobo ea Tšoaetso XueFeng Wang ORCID iD: 0000-0001-8854-275X
Nako ea poso: Mar-17-2020
